Research in the Luger Lab focuses on the structural biology of genome organization. We aim to understand the fundamental impact of chromatin architecture on genome-related processes such as regulated gene transcription, DNA replication, and DNA repair. We study the interaction of nucleosomes with nuclear factors, and on structural and mechanistic aspects of the cellular machinery that assembles and disassembles chromatin during transcription, replication, and DNA repair. The evolution of eukaryotic chromatin structure is investigated through structural studies of archaeal chromatin and chromatin-associated factors.
The main biochemistry lab (approximately 3,700 sqft.) is located on the second floor, in the blue A-wing, and offers a view of the beautiful Colorado Front Range. It has room for 20 researchers.
We operate five HPLC / FPLC protein purification stations, two preparative gel electrophoresis stations, centrifuges, incubator/shakers, and all other necessary equipment for protein expression and purification. We have access to the centralized Keck protein purification facility at Colorado State University in Fort Collins, and the lab runs its own baculovirus expression facility.
Our state-of-the art x-ray and crystallization facility (expertly led by Dr. Dave McKay) are located in the basement of the same building. A crystallization robot allows us to perform high-throughput screening of crystallization conditions. Our Asylum Atomic Force Microscope is capable of imaging in liquid and air and is also used for force extension curves. We operate a Beckman analytical ultracentrifuge (equipped with fluorescence scanning optics), and we have access to fluorimeters, CD, ITC, and various phosphor imagers (all housed in the Biochemistry Core Facility, in JSCBB, led by Dr. Annette Erbse. Hydrogen-Deuterium exchange experiments are performed in the CU Mass spectrometry facility.